The final two kits from the Y-DNA recruiting effort have completed, about a month earlier than expected. For the details on the first three kits, see this post.
Kit #4 was for a known descendant of Nicholas Ackley through his son Samuel. This represents something new for the project; all current members who have a known connection to Nicholas are descendants of either his son John or his son James. The quick and dirty tree for this tester (again, leaving off the most recent generations to protect his privacy) looks like this (sorry for the small font):
As expected, the kit from the descendant of Nicholas Ackley through his son Samuel matched all of the other known descendants of Nicholas. The fifth kit also matched every one of the known descendants of Nicholas, so he and anyone else who is a descendant of Lewis Ackley of Otsego County, New York can focus their research on finding their connection to Nicholas. Interestingly, as mentioned in part 1, one of the other new members is a descendant of another Ackley from Otsego County, James D. Ackley, born in 1799. Certainly more research is needed, but perhaps James D. and Lewis were closely related; their birth years are close enough that they could be brothers or close cousins.
The relationship between genetic distance and relatedness is summarized in the table below (same table in part 1). Since the new testers all took a Y-37 test, we will make our comparisons at the the 37 marker level.